Molecular Biology Techniques

Molecular Biology Techniques PDF Book Detail:
Author: Sue Carson
Publisher: Academic Press
ISBN: 0123855446
Size: 48.18 MB
Format: PDF, Kindle
Category : Medical
Languages : en
Pages : 200
View: 7506

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Book Description: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Unraveling Dna

Unraveling DNA PDF Book Detail:
Author: Michael R. Winfrey
Publisher: Benjamin-Cummings Publishing Company
Size: 43.88 MB
Format: PDF, ePub
Category : Science
Languages : en
Pages : 369
View: 6154

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Book Description: This manual encompasses an integrated series of molecular biology laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri. KEY TOPICS: The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. The manual is based on one of nature's most fascinating biological phenomenon: the biological production of light. This results in a recurrent theme of interest and makes the project very relevant to interdisciplinary topics such as fish symbiosis, biochemistry, biophysics, etc. Includes instruction in the basic techniques of modern molecular biology: DNA isolation and analysis, DNA restriction, agarose gel electrophoresis, ligations, transformation of recombinant DNA, preparation and screening a genomic library, restriction mapping, Southern blotting, hybridization, DNA sequencing, pulsed field gel electrophoresis. MARKET: Designed for a one semester course in Molecular Biology. Also appropriate for a molecular biology component of Microbial Genetics, Genetics, Biochemistry, or Advanced Microbiology courses.

Principles Of Biology

Principles of Biology PDF Book Detail:
Author: Virginia G. Latta
Publisher: Hunter Books
ISBN: 9780887251504
Size: 59.13 MB
Format: PDF, Kindle
Category : Biology
Languages : en
Pages : 218
View: 1844

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Book Description: This manual has proved to be especially popular for introductory biology labs emphasizing a molecular-cellular approach. The 12 exercises are ideal for the quarter length or semester program and are adaptable for use with most textbooks. Designed for majors and non-majors, the manual begins with the fundamentals. For students with little or no background, the first two exercises focus on developing laboratory skills. Exercises are consistently organized: theory relates lab experiences with concepts presented in lecture; objectives summarize skills and concepts to be mastered; materials and equipment needed for the exercise are an aid for instructors; procedures are described step-by-step; and detachable lab reports are provided for hand-ins. All exercises have been thoroughly class-tested. The manual is self-contained and adaptable for use with most textbooks. Highlights include numerous illustrations, many with color added for clarity; an appendix on the metric system for hand student reference; and 16 pages of extra graph paper. A plus for instructors is the appendix with instructions for preparing solutions, reagents, and materials needed. An answer key for lab reports is available on adoption.

Exercises For The Zoology Laboratory

Exercises for the Zoology Laboratory PDF Book Detail:
Author: David G. Smith
ISBN: 9780895825155
Size: 67.31 MB
Format: PDF, ePub
Category : Zoology
Languages : en
Pages : 126
View: 5547

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Book Description:

Experimental Developmental Biology

Experimental Developmental Biology PDF Book Detail:
Author: Laura R. Keller
Publisher: Academic Press
ISBN: 9780124039704
Size: 57.22 MB
Format: PDF, Docs
Category : Science
Languages : en
Pages : 114
View: 4800

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Book Description: Experimental Developmental Biology: A Laboratory Manual is designed for use in college-level laboratory courses in developmental biology. It offers challenging experiments for students to perform as independent investigators as they probe developmental processes in living embryos at the organizational, cellular, and subcellular levels. * Combines classical embryology with modern experimental methods * Provides numerous in-depth experiments in each exercise that focus on a single species of an organism * Concentrates on the living embryos of sea urchins, frogs, chicks, Drosophila, and sponges * Covers the procedures for gel electrophoresis and microscopy * Assembles essential references for background and further study * Offers guidelines for writing lab notes and reports * Contains an extensive preparer's guide to show students how to set up each lab * Outlines the theory of optics

Exercises For The Anatomy Physiology Laboratory

Exercises for the Anatomy   Physiology Laboratory PDF Book Detail:
Author: Erin C. Amerman
Publisher: Morton Publishing Company
ISBN: 1617319406
Size: 61.25 MB
Format: PDF, Kindle
Category : Science
Languages : en
Pages : 368
View: 4956

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Book Description: This concise, inexpensive, black-and-white manual is appropriate for one- or two-semester anatomy and physiology laboratory courses. It offers a flexible alternative to the larger, more expensive laboratory manuals on the market. This streamlined manual shares the same innovative, activities-based approach as its more comprehensive, full-color counterpart, Exploring Anatomy & Physiology in the Laboratory, 3e.