Aqueous Two Phase Partitioning

Aqueous Two Phase Partitioning PDF Book Detail:
Author: Boris Y. Zaslavsky
Publisher: CRC Press
ISBN: 9780824794613
Size: 53.87 MB
Format: PDF, ePub, Mobi
Category : Science
Languages : en
Pages : 656
View: 4716

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Book Description: Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.

Partitioning In Aqueous Two Phase System

Partitioning In Aqueous Two     Phase System PDF Book Detail:
Author: Harry Walter
Publisher: Elsevier
ISBN: 0323142915
Size: 66.80 MB
Format: PDF, ePub, Mobi
Category : Science
Languages : en
Pages : 730
View: 6827

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Book Description: Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.

Aqueous Two Phase Systems

Aqueous Two phase Systems PDF Book Detail:
Author: Rajni Hatti-Kaul
Publisher: Springer Science & Business Media
ISBN: 1592590284
Size: 77.17 MB
Format: PDF, ePub
Category : Science
Languages : en
Pages : 440
View: 1050

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Book Description: In Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications, all presented in set-by-step detail to ensure easy reproducibility and robust results. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Techniques for in situ product recovery during biocatalytic processes and for polymer-polymer systems in organic solvents are also presented. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

Aqueous Two Phase Systems

Aqueous Two Phase Systems PDF Book Detail:
Author: Harry Walter
Publisher: Elsevier
ISBN: 9780121821296
Size: 70.68 MB
Format: PDF
Category : Science
Languages : en
Pages : 725
View: 6898

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Book Description: General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.

The Science And Application Of Aqueous Two Phase Systems And Liquid Liquid Phase Separation In Biotechnology And Bioengineering

The Science and Application of Aqueous Two Phase Systems and Liquid Liquid Phase Separation in Biotechnology and Bioengineering PDF Book Detail:
Author: John Paul Frampton
Publisher: Frontiers Media SA
ISBN: 288963356X
Size: 65.58 MB
Format: PDF, Mobi
Category :
Languages : en
Pages :
View: 384

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Book Description: The phase separation of incompatible liquids has been a topic of significant importance in chemical and industrial engineering for many years. Well-understood examples of this phenomenon include the phase separation of oil with water and the phase separation of non-polar organic solvents with water. Similar behavior is observed when aqueous solutions of two or more incompatible polymers or polymers and salts are mixed. In these mixtures (referred to as aqueous two-phase systems), the separated phases are composed mostly of water. Aqueous two-phase systems have been used extensively for the extraction of high-value biological products from mixtures of biological materials. In recent years, aqueous two-phase systems have also found increased use as materials for streamlining and improving the capabilities of cell and molecular assays, and for the design of advanced cell culture systems. Similar behavior of biological materials in living systems has also been observed, with emerging roles in cell physiology.

Aqueous Two Phase Systems For Bioprocess Development For The Recovery Of Biological Products

Aqueous Two Phase Systems for Bioprocess Development for the Recovery of Biological Products PDF Book Detail:
Author: Marco Rito-Palomares
Publisher: Springer
ISBN: 3319593099
Size: 32.77 MB
Format: PDF, ePub, Mobi
Category : Technology & Engineering
Languages : en
Pages : 212
View: 3790

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Book Description: This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.

Aqueous Two Phase Systems

Aqueous Two phase Systems PDF Book Detail:
Author: Vangelis Anatolijs Xanthopoulos
Publisher:
ISBN: 9781536142419
Size: 79.88 MB
Format: PDF, Mobi
Category :
Languages : en
Pages : 103
View: 3103

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Book Description: Aqueous Two-Phase Systems (ATPS) have been used since their discovery for the isolation of a large number of different biological materials, such as animal and plant cells, microorganisms, viruses, cellular organelles such as chloroplasts, mitochondria, membrane vesicles, and also in the purification of proteins and nucleic acids. In Aqueous Two-Phase Systems: Properties, Functions and Advantages, the authors begin by discussing the benefits of these systems.The following chapter presents the main forms of the purification of lipases from aqueous biphasic systems, addressing and discussing the fundamentals for the formation, use and optimization of these systems applied in the biotechnological context. The authors address the basis of aqueous-biphasic systems, aqueous two-phase systems for enzymatic purification, purification of lipases and combined uses of aqueous two-phase systems.The most important experimental parameters that affect the efficiency of metal ions extraction in aqueous PEG-based two-phase systems are examined to provide a starting point in the design of a suitable system for the extraction of metal ions. Compared to the conventional solvent extraction, which uses toxic, flammable and volatile organic solvents and can be quite expensive, the extraction in aqueous PEG-based two-phase systems is considered a more environmental friendly and economically viable method.Also in this compilation, an overview about application of ATPSs in microstructured devices is provided. Microstructured devices offer potential benefits due to well-defined high specific interfacial areas available for heat and mass transfer. These areas increase transfer rate and enhance yield, selectivity and process control. The application of ATPSs in Micro Total Analysis Systems is described as well.

Heme Chlorophyll And Bilins

Heme  Chlorophyll  and Bilins PDF Book Detail:
Author: Alison Smith
Publisher: Springer Science & Business Media
ISBN: 1588291111
Size: 70.73 MB
Format: PDF, Docs
Category : Science
Languages : en
Pages : 340
View: 6717

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Book Description: Since the discipline of handling and manipulating tetrapyrroles has been in practice for the past century, the material published in this field is seldom current and often does not include technical tips that can greatly influence the success of the provided methods for preparation and analysis. Handbooks have been published over the years that are now out-of-date and are theoretical rather practical in their orientation. In Heme, Chlorophyll, and Bilins: Methods and Protocols, scientists in the field provide detailed descriptions of basic protocols and technology for the study of chlorophyll, heme, and related molecules, including technical tips and suggestions to avoid common pitfalls. Simpler, more traditional methods, as well as the more modern and state-of-the-art procedures have been maintained, updated, and refined. This text is a current and comprehensive overview of chemical, biophysical, and molecular biological topics for accessible use by both experienced and novice laboratory researchers.